甘油三酯类辅料分离和分析技术进展

朱文星,杨锐,闫中天,汤龙,孙会敏

中国药学杂志 ›› 2016, Vol. 51 ›› Issue (14) : 1224-1229.

PDF(1168 KB)
PDF(1168 KB)
中国药学杂志 ›› 2016, Vol. 51 ›› Issue (14) : 1224-1229. DOI: 10.11669/cpj.2016.14.016
综述

甘油三酯类辅料分离和分析技术进展

  • 朱文星1,2,杨锐 1,闫中天1,汤龙1,孙会敏1*
作者信息 +

Development of Methods for Separation and Analysis of Triacylglycerols

  • ZHU Wen-xing1,2, YANG Rui1, YAN Zhong-tian1, TANG Long1, SUN Hui-min1*
Author information +
文章历史 +

摘要

随着现代药物递送系统的发展,甘油三酯(triacylglycerols,TAGs)类脂质辅料在药物制剂中的应用日益广泛。由于TAGs的性质与其结构组成具有重要联系,为指导TAGs类药用辅料的应用,应对其进行严格的质量控制,然而,TAGs组成复杂使得有效分析手段缺失。笔者对TAGs分离分析常用的薄层色谱法、固相萃取法、气相色谱法、液相色谱法和二维液相色谱法以及超高效合相色谱法等进行了较为全面的综述,比较了各技术的优缺点和适用范围;并结合自身研究认为超高效合相色谱是TAGs最有效的分析方法之一。

Abstract

As one kind of lipid excipients, triacylglycerols (TAGs) are being more and more widely used in pharmaceutics with the development of advanced drug delivery systems.As their characteristics are mainly determined by their structures and compositions, strict quality control ought to be performed on TAGs, in order to guide their use in pharmaceutics. However, because of their complex compositions, no qualtity standard is set for TAGs in Ch.P currently.In this paper, we reviewed the methods for the separation and analysis of TAGs, includingthin-layer chromatog raphy, solid-phase extraction, gas chromotography, liquid chromatography, two-dimensional liquid chromatography,and ultraperformance convergence chromatography, and summarized their advantages and disadvantages.The application scope of each method was also overviewed in this review. Besides that we also pointed outthat according to our own study the newly emerged ultraperformance convergence chromatography would be one of the best methods to analyze TAGs.

关键词

甘油三酯 / 分离分析 / 二维液相色谱 / 超高效合相色谱

Key words

triacylglycerols / separation and analysis / two dimensional liquid chromatography / ultraperformance convergence chromatography

引用本文

导出引用
朱文星,杨锐,闫中天,汤龙,孙会敏. 甘油三酯类辅料分离和分析技术进展[J]. 中国药学杂志, 2016, 51(14): 1224-1229 https://doi.org/10.11669/cpj.2016.14.016
ZHU Wen-xing, YANG Rui, YAN Zhong-tian, TANG Long, SUN Hui-min. Development of Methods for Separation and Analysis of Triacylglycerols[J]. Chinese Pharmaceutical Journal, 2016, 51(14): 1224-1229 https://doi.org/10.11669/cpj.2016.14.016
中图分类号: R917   

参考文献

[1] ROSIAUX Y, JANNIN V, HUGHES S, et al. Solid lipid excipients-matrix agents for sustained drug delivery . J Controlled Release, 2014, 188:18-30.
[2] BECKER K, SALAR-BEHZADI S, ZIMMER A. Solvent-free melting techniques for the preparation of lipid-based solid oral formulations . Pharm Res, 2015, 32(5):1519-1545.
[3] DAI W, LIU X Y, LI J H, et al. Development of the novel cationic nanoemulsion drug system. Chin Pharm J(中国药学杂志), 2013, 48(23):1980-1984.
[4] CHAWLA V, SARAF S A. Glyceryl behenate and its suitability for production of aceclofenac solid lipid nanoparticles . J Am Oil Chem Soc, 2010, 88(1):119-126.
[5] LIN W T, LONG X Y, WU H Y, et al. Drug distribution during in vitro lipolysis of SNEDDSs containing griseofulvin . Chin Pharm J(中国药学杂志), 2015, 50(6):512-520.
[6] CHEN J , LI B , WU D W, et al. Preparation and evaluation of docetaxel nanoemulsion . Chin Pharm J(中国药学杂志), 2010, 45(7):534-538.
[7] CHRISTIE W W, HAN X. Lipid Analysis (Fourth Edition) . Bridgwater:Woodhead Publishing, 2012.
[8] EZANKA T, SIGLER K. Separation of enantiomeric triacylglycerols by chiral-phase HPLC . Lipids, 2014, 49(12):1251-1260.
[9] FUCHS B, SUSS R, TEUBER K, et al. Lipid analysis by thin-layer chromatography--a review of the current state . J Chromatogr A, 2011, 1218:2754-2774.
HAMILTON J, COMAI K. Separation of neutral lipids and free fatty acids by high-performance liquid chromatography using low wavelength ultraviolet detection . J Lipid Res, 1984, 25(10):1142-1148.
WEI F, HU N, LV X, et al. Quantitation of triacylglycerols in edible oils by off-line comprehensive two-dimensional liquid chromatography-atmospheric pressure chemical ionization mass spectrometry using a single column . J Chromatogr A, 2015, 1404:60-71.
LESELLIER E, LATOS A, DE OLIVEIRA A L. Ultra high efficiency/low pressure supercritical fluid chromatography with superficially porous particles for triglyceride separation . J Chromatogr A, 2014, 1327:141-148.
DUGO P, CACCIOLA F, KUMM T, et al. Comprehensive multidimensional liquid chromatography:theory and applications . J Chromatogr A, 2008, 1184:353-368.
HOLCAPEK M, OVCACIKOVA M, LISA M, et al. Continuous comprehensive two-dimensional liquid chromatography-electrospray ionization mass spectrometry of complex lipidomic samples . Anal Bioanal Chem, 2015, 407(17):5033-5043.
DONG X, ZHONG J, WEI F, et al. Triacylglycerol composition profiling and comparison of high-oleic and normal peanut oils . J Am Oil Chem Soc, 2015, 92(2):233-242.
HU J, WEI F, DONG X Y, et al. Characterization and quantification of triacylglycerols in peanut oil by off-line comprehensive two-dimensional liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry . J Sepsci, 2013, 36(2):288-300.
SANDRA K S P. The opportunities of 2D-LC in the analysis of monoclonal antibodies . Bioanalysis, 2015, 7(22):2843-2847.
WEI F, JI S X, HU N, et al. Online profiling of triacylglycerols in plant oils by two-dimensional liquid chromatography using a single column coupled with atmospheric pressure chemical ionization mass spectrometry . J Chromatogr A, 2013, 1312:69-79.
LSA M, HOLAPEK M. Characterization of triacylglycerol enantiomers using chiral HPLC/APCI-MS and synthesis of enantiomeric triacylglycerols . Anal Chem, 2013, 85(3):1852-1859.
EZANKA T, NEDBALOVL, SIGLER K. Comparative analysis of triacylglycerols from different stichococcus strains by RP-HPLC/APCI-MS and chiral HPLC . J App Phycol, 2014, 27(2):685-696.
IWASAKI Y, YASUI M, ISHIKAWA T, et al. Optical resolution of asymmetric triacylglycerols by chiral-phase high-performance liquid chromatography . J Chromatogr A, 2001, 905:111-118.
SHEN Z, WIJESUNDERA C. Evaluation of ethanolysis with immobilized candida antarctica lipase for regiospecific analysis of triacylglycerols containing highly unsaturated fatty acids . J Am Oil Chem Soc, 2006, 83(11):923-927.
NURMELA K V, SATAMA L T. Quantitative analysis of triglycerides by high-performance liquid chromatography using non-linear gradient elution and flame ionization detection . J Chromatogr A, 1988, 435:139-148.
ROMBAUT R, DE CLERCQ N, FOUBERT I, et al. Triacylglycerol analysis of fats and oils by evaporative light scattering detection . J Am Oil Chem Soc, 2009, 86(1):19-25.
HU N, WEI F, LV X, et al. Profiling of triacylglycerols in plant oils by high-performance liquid chromatography-atmosphere pressure chemical ionization mass spectrometry using a novel mixed-mode column . J Chromatogr B Analyt Technol Biomed Life Sci, 2014, 972:65-72.
BECCARIA M, SULLINI G, CACCIOLA F, et al. High performance characterization of triacylglycerols in milk and milk-related samples by liquid chromatography and mass spectrometry . J Chromatogr A, 2014, 1360:172-187.
FASCIOTTI M, PEREIRA NETTO A D. Optimization and application of methods of triacylglycerol evaluation for characterization of olive oil adulteration by soybean oil with HPLC-APCI-MS-MS . Talanta, 2010, 81(3):1116-1125.
GMEZ-ARIZA J, ARIAS-BORREGO A, GARC A-BARRERA T, et al. Comparative study of electrospray and photospray ionization sources coupled to quadrupole time-of-flight mass spectrometer for olive oil authentication . Talanta, 2006, 70(4):859-869.
HAKOVA E, VRKOSLAV V, MIKOVA R, et al. Localization of double bonds in triacylglycerols using high-performance liquid chromatography/atmospheric pressure chemical ionization ion-trap mass spectrometry . Analyt Bioanal Chem, 2015, 407(17):5175-5188.
BAIOCCHI C, MEDANA C, DAL BELLO F, et al. Analysis of regioisomers of polyunsaturated triacylglycerols in marine matrices by HPLC/HRMS . Food Chem, 2015, 166:551-560.
HERSLF F B, PODLAHA O, T REG R D B. HPLC of triglycerides . J Am Oil Chem Soc, 1979, 56(9):864-866.
HIERRO M, TOMAS M, FERNANDEZ-MARTIN F, et al. Determination of the triglyceride composition of avocado oil by high-performance liquid chromatography using a light-scattering detector . J Chromatogr A, 1992, 607:329-338.
LPEZ-HERNNDEZ A, TORRES C F, GARCA H S, et al. Method for analysis of TAG formed by reaction of fish oil with hydrogenated soybean oil . J Am Oil Chem Soc, 2004, 81(8):743-747.
SOLAESA G, BUCIO S L, SANZ M T, et al. Characterization of triacylglycerol composition of fish oils by using chromatographic techniques . J Oleo Sci, 2014, 63(5):449-460.
KALIKOVA K, SLECHTOVA T, VOZKA J, et al. Supercritical fluid chromatography as a tool for enantioselective separation; a review . Anal Chim Acta, 2014, 821:1-33.
ASHRAF-KHORASSANI M, YANG J, RAINVILLE P, et al. Ultrahigh performance supercritical fluid chromatography of lipophilic compounds with application to synthetic and commercial biodiesel . J Chromatogr B Anal Technol Biomed Life Sci, 2015, 983-984(1):94-100.
GRAND-GUILLAUME PERRENOUD A, VEUTHEY J L, GUILLARME D. Comparison of ultra-high performance supercritical fluid chromatography and ultra-high performance liquid chromatography for the analysis of pharmaceutical compounds . J Chromatogr A, 2012, 1266:158-167.
TAO Y, PAULA R, STOLKER A A, et al. Simultaneous determination of seven gestagens in kidney fats by ultra performance convergence chromatography tandem mass spectrometry . J Chromatogr B Anal Technol Biomed Life Sci, 2015, 988:143-148.
PERRENOUD A G-G, BOCCARD J, VEUTHEY J L, et al. Analysis of basic compounds by supercritical fluid chromatography:attempts to improve peak shape and maintain mass spectrometry compatibility . J Chromatogr A, 2012, 1262:205-213.
ZHOU Q, GAO B, ZHANG X, et al. Chemical profiling of triacylglycerols and diacylglycerols in cow milk fat by ultra-performance convergence chromatography combined with a quadrupole time-of-flight mass spectrometry . Food Chem, 2014, 143:199-204.
ASHRAF-KHORASSANI M, ISAAC G, RAINVILLE P, et al. Study of ultra high performance supercritical fluid chromatography to measure free fatty acids with out fatty acid ester preparation . J Chromatogr B Anal Technol Biomed Life Sci, 2015, 997:45-55.

基金

国家科技重大专项重大新药创制资助项目(2015ZX09303001)
PDF(1168 KB)

Accesses

Citation

Detail

段落导航
相关文章

/